To prove that the developed drug is working ( either biosimilar or discovery) , having a proper, viable, cheap , reproducible in-vitro bio-assay is crucial.
1. Currently the backbone or workhorse of in-vitro bio-assays are ELISAs.
To establish an ELISA basic raw materials are antigens ( our case drug targets ), antibody and conjugated antibody, developing agents.
Several thousand suppliers are there for antibodies and developing agents. But getting a good quality targets is always cumber some.
You have to spend considerable amount of time to procure good targets and store it.
2. If ELISA is not workable, then next way of approach is cell based assays.
1. Currently the backbone or workhorse of in-vitro bio-assays are ELISAs.
To establish an ELISA basic raw materials are antigens ( our case drug targets ), antibody and conjugated antibody, developing agents.
Several thousand suppliers are there for antibodies and developing agents. But getting a good quality targets is always cumber some.
You have to spend considerable amount of time to procure good targets and store it.
2. If ELISA is not workable, then next way of approach is cell based assays.
How to read the final data. The common principle is absorption of Light.
The suppliers of these instruments are
Who supplies Drug targets and Assay kits.
- Life Technologies
- Perkin Elmer
- Promega
- E Merck
- GE Health care
- Thermo
- Sigma
But keep in mind. If you want to reduce cost developing own in-house kits are advisable if you have time, resources and kowled
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